Premium
Towards a new therapy concept for acute microbial karatides, including Acanthamoebae
Author(s) -
Storsberg J.,
Schmidt C.,
Plog C.,
Höfer P.,
Klöpzig S.,
Rehfeldt S.,
Sel S.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2017.0t076
Subject(s) - staphylococcus aureus , pseudomonas aeruginosa , microbiology and biotechnology , in vitro , inoculation , staining , biology , bacterial growth , pseudomonas , bacteria , medicine , immunology , pathology , biochemistry , genetics
Purpose The purpose of this study was to develop novel therapeutic approaches for the management and treatment of microbial keratitides, especially pathologies resulting from Acantamoeba castellanii inoculations. Methods Growth inhibitory effects of plasma‐induced reactive species on cultures of Staphylococcus aureus, Pseudomonas aeruginosa, Acantamoeba castellanii, and MRSA were assayed for in in an in‐vitro setting. Human donor corneas were inoculated and cultured with cultures of above pathogens, followed by staining of cells and microscopic analysis. Results Our results show a substantial inhibitory effect of plasma‐induced reactive species on the growth and migration of the pathogens tested. Conclusions The effects observed provide foundation for the development of new avenues for the management and therapy of microbial keratitides.