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Effects of short oxidative stress exposure on lens epithelial cells
Author(s) -
D'antin J.C.,
Barraquer R.I.,
Michael R.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2017.0s073
Subject(s) - oxidative stress , hydrogen peroxide , posterior capsule opacification , lens (geology) , capsule , andrology , ophthalmology , chemistry , biology , medicine , biochemistry , botany , visual acuity , paleontology , phacoemulsification
Purpose To observe the reactions of lens epithelial cells (LECs) to a short exposure of an elevated oxidative stress. To help find future treatments for the prevention of posterior capsule opacification. Using cultured human lens capsules as an experimental model and hydrogen peroxide (H2O2) as the stress inducer. Methods Lens capsule‐ciliary body complexes were extracted from human donor eyes. Samples were exposed to 30 mM H 2 O 2 for 5 min (n = 8) or used as untreated controls (n = 9). H2O2 was applied after lens extraction through intercapsular irrigation using a silicone irrigation ring. Samples were cultured on average for 30 days, during which dark field and lateral illumination photos were taken every 2–3 days. These photos were used to observe and quantify, time until cellular growth and subsequent confluence on the posterior capsule. Three of the controls were not cultured, in order to observe starting conditions. Results All control samples showed signs of normal cellular growth on the posterior capsule on average by day 6. LEC proliferation and migration was not observed on H 2 O 2 samples until day 22. Four H 2 O 2 samples showed signs of growth. The overall delay of cell growth compared to control was significant (H 2 O 2 p < 0.001). Until day 29 none of the exposed samples that had shown growth reached confluence. Half of controls were confluent on day 10, 83% on day 20 and all by day 26. The average cellular migration speed of controls and H 2 O 2 samples were very similar, being 3.2 and 3.0 (mm 2 /day) respectively. Conclusions Exposure of lens epithelial cells to an elevated concentration of H 2 O 2 for a short period of time does not seem to lead to immediate cell death as expected, but rather arrests the cell cycle. Surprisingly, after cells have recovered from the oxidative stress, they grow at a normal rate.