Premium
Recombinant human heat shock protein 27 can inhibit ultraviolet B‐induced differentiation in pterygial‐derived fibroblast
Author(s) -
Kim J.Y.,
Moon C.H.,
Shin J.A.,
Kang S.S.,
Kim E.S.,
Tchah H.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2017.0s035
Subject(s) - fibroblast , myofibroblast , western blot , recombinant dna , microbiology and biotechnology , conjunctiva , medicine , chemistry , immunology , pathology , biology , fibrosis , in vitro , biochemistry , gene
Purpose To investigate the effect of recombinant human heat shock protein 27 (rh HSP 27) on the differentiation of human pterygial‐derived fibroblast. Methods Human conjunctival and pterygial fibroblasts were isolated and cultured from specimens of normal conjunctiva and pterygium head, which were donated during pterygium excision. Cultured conjunctival and pterygial fibroblasts were exposed to 20 mJ /cm2 of ultraviolet‐B ( UVB ) irradiation with and without 4 ug/ mL rh HSP 27 pre‐treatment. Western blot analyses of α ‐smooth muscle actin ( α ‐ SMA ), a marker for myofibroblast, were performed in each group and quantified using Image Gauge 4.0 software. The ratios of α ‐ SMA / GAPDH and phosphorylated HSP 27 / GAPDH were compared. Results The α ‐ SMA level in Pterygial fibroblasts showed 2.85 ± 0.11 fold greater than that of normal conjunctival fibroblasts (P < 0.001). The level of α ‐ SMA was increased by 1.92 ± 0.02 times (P < 0.001), when normal conjunctival fibroblasts were exposed to UVB irradiation without rh HSP 27 pre‐treatment. However, when normal conjunctival fibroblasts were exposed to UVB irradiation with rh HSP 27 pre‐treatment, the fibroblasts demonstrated 0.82 ± 0.04 fold smaller α ‐ SMA level than control did (P = 0.04). Conclusions The rh HSP 27 revealed inhibitory effects on the UVB irradiation‐induced differentiation from normal conjunctival fibroblasts to pterygial myofibroblast. The rh HSP 27 can be a preventive treatment against pterygium growth.