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Aqueous inflammatory proteasome in open angle glaucoma in Caucasian patients
Author(s) -
Pantalon A.,
Feraru C.,
Constantinescu D.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2017.04127
Subject(s) - medicine , glaucoma , ophthalmology , optic nerve , open angle glaucoma , intraocular pressure , interleukin 8 , proinflammatory cytokine , cytokine , inflammation
Purpose Primary open‐angle glaucoma is characterized by loss of retinal ganglion cells and their axons, resulting in optic nerve cupping and visual field loss. Until now, no specific cause was attributed to POAG development, but multiple pathogenic theories have been approached, beside IOP elevation and aging.The aim of this study was to assess the inflammatory and immune dysregulation theory in POAG . Methods We included in a cross‐sectional study 40 eyes, from 40 patients: 16 eyes with POAG and 24 eyes from healthy subjects. Aqueous was collected during conventional cataract surgery. 21 inflammatory markers were quantified and compared between groups using a Luminex ® Performance Assay multiplex kit based on flowcytometric methods. Results Mean age in POAG group was 75.69 ± 5.54 years vs 72.33 ± 11.26 years in controls (p = 0.23). Mean IOP in healthy controls was 14.21 ± 2.68 mmHg compared to 18.19 ± 4.3 mmHg in glaucoma patients, controlled by 3 ± 0.87 topical substances. Mean MD level in POAG group was ‐13.59+/‐9.35 dB , whereas PSD mean level was 4.25 ± 4.22dB. Cytokines expression in glaucoma patients compared to healthy controls was found significantly different for CXCL 5 (p = 0.008), CXCL 8 (p = 0.048), IL ‐1 α (p = 0.005), IL ‐2 (p = 0.015) and TNF α (p = 0.041). Therefore, a prediction statistical model for these cytokines was created. All markers point out a common inflammatory pathway that triggeres TNF α release. A mathematical model proved that CXCL 5, CXCL 8, IL ‐1 α and IL ‐2 can accurately predict TNF α level in this study ( r square = 0.842, p = 0.000). Conclusions Our results show that in POAG patients there is an increased production of inflammatory cytokines in aquoeous humor. Moreover our statistical predictions point out TNF α molecule and its signalling pathways as the determinant pathogenic pathway involved in the inflammatory compound of POAG caucasian patients.