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Proteinics study: Relevance and interest for screening, follow‐up, etiopathogenesis of AMD
Author(s) -
Gonzalez C.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2017.03336
Subject(s) - medicine , group b , macular degeneration , biomarker , stage (stratigraphy) , ophthalmology , fundus (uterus) , fluorescein angiography , atrophy , visual acuity , pathology , gastroenterology , paleontology , biochemistry , chemistry , biology
Purpose To evaluate Proteinics study impact on AMD diagnosis, screening, follow‐up, etiopathogenesis. Methods AMD : 40 patients,16 men,24 women,30 with AMD .4 Groups A,B,C,T. A:10 first stage AMD patients,B:10 Atrophy AMD patients(predominant atrophic areas),C:10 patients with Neovascular AMD ,T:10 normal patients,control group. Ophthalmologic exam included ETDRS visual acuity( VA ),complete ophthalmic examination,Fundus examination,autofluorescence imaging( FAF ),(RegionFinderSoftware),optical coherence tomography( HRA Spectral Domain OCT )( OCT enFace software, M‐S software),Fluorescein angiography( FA )and ICG when Neovascular complication. Proteinics Study: Blood tests and analysis, proteinics qualitative and quantitative analysis,all the same for all patients,whatever group. Blood test is done during ophthalmologic exam. Plasma congelation,then Elisa technic,3 stages(SimpleStep Elisa kits)for detection and quantification of IL 1 β , IL 6, IL 10, TNF α ,tau protein. Classic Elisa technic,2stages,for Amyloid peptid Aß1‐40.Each sample quadruplicate; Micro BCA analysis. Results Analysis will determine proteinics quantitative values in each group of patients, and proportion, characterization, singularity of each of them: group A and C:similar results, higher for C; group T and B:similar results, lower for B. So, characterization, prevalence, specifics of and for each group, therefore a proteinics profile. Proteinics study'evaluation, identification, classification in AMD patients, and especially A, B, C groups allow AMD screening, follow‐up, particularly according to AMD type and stage. Proteinics study may have biomarker feature and let AMD prevention Conclusions Proteinics study enhance AMD characterization, allow better diagnosis, follow‐up, screening. Interrelations, correlations between AMD and Proteinics lead to better etiopathogenesis understanding and therapeutics prospects.