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Flat‐mount of retina, including preparation of choroid and iris
Author(s) -
Andre H.
Publication year - 2017
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2017.01343
Subject(s) - choroid , iris (biosensor) , retina , retinopathy of prematurity , ophthalmology , macular degeneration , medicine , diabetic retinopathy , retinal , neovascularization , biology , biometrics , angiogenesis , computer science , neuroscience , endocrinology , pregnancy , genetics , computer security , diabetes mellitus , gestational age
Summary Flatmount methods have been applied to multiple animal models to grant a panoramic view of specific tissues. Mouse models of retinopathy of prematurity (oxygen‐induced retinopathy) and age‐related macular degeneration (laser‐induced choroidal neovascularization and iodate‐induced geographic atrophy) have generally been assayed from whole‐tissue flatmount studies. We have recently developed a method for mouse iris flatmounts as a model of rubeosis iridis, a clinical diagnosis associated with proliferative diabetic retinopathy and neovascular glaucoma. The aim for the course is to illustrate technical aspects of mouse retinal, choroidal, and iris flatmounts, as well as methods for analysis and interpretation of results.