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Vitreous and serum Hsp 70 levels in rhegmatogenous retinal detachment
Author(s) -
SozenDelil F.I.,
Cekic O.,
Haklar G.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0658
Subject(s) - vitrectomy , epiretinal membrane , retinal detachment , pars plana , ophthalmology , medicine , retinal , heat shock protein , visual acuity , andrology , chemistry , biochemistry , gene
Purpose Heat shock proteins (Hsp) are ubiquitous proteins that protect cells from different kinds of stress. Retinal detachment (RD) is a critical eye situation after that death of photoreceptors by apoptosis and necrosis occurs. Despite the best anatomical result following the surgery visual acuity may not improve. The objective of the present study was to detect possible correlations of Hsp 70 levels between vitreous and serum of the patients with RD. Methods Totally 15 eyes of 15 patients were included. The study group consisted of 11eyes with rhegmatogenous RD and the control group consisted of 4 eyes with macular hole or epiretinal membrane without RD. All eyes underwent pars plana vitrectomy. Vitreous and serum samples were obtained at the time of vitrectomy. The levels of Hsp 70 were analyzed using Enzyme linked immuno‐sorbent assay. Total vitreous proteins and total serum proteins were also measured. Mann–Whitney U and Spearman correlation tests were performed for the statistics. The significance was set to <0.05. Results In sum total ( n = 15), higher mean vitreous Hsp 70 level (±standard deviation) were detected in eyes than that of serum (184.41 ± 154.4 pg/ml vs. 85.43 ± 27.47 pg/ml, p < 0.001). In the study group, vitreous Hsp 70 levels are higher than those of serum (209.33 ± 175.12 pg/ml vs. 83.95 ± 26.95 pg/ml, p < 0.001) as well as serum and vitreous Hsp 70 levels were not correlated (p > 0.05). In the control group, serum and vitreous Hsp 70 levels were similar (89.49 ± 32.72 pg/ml vs. 115.87 ± 22.05 pg/ml, p > 0.05), and serum and vitreous Hsp 70 levels were not correlated (p > 0.05). Conclusions Elevated vitreous Hsp 70 levels in the eyes with rhegmatogenous RD could be due to increased endogenous synthesis to prevent stressed retina from apoptosis. Acknowledgement This project was supported by Marmara University, Scientific Research Projects Committee.