Premium
Vitreous and subretinal VEGF levels in fresh rhegmatogenous retinal detachment
Author(s) -
SozenDelil F.I.,
Cekic O.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0651
Subject(s) - vitrectomy , pars plana , retinal detachment , ophthalmology , medicine , retinal , vascular endothelial growth factor , retinal pigment epithelium , retina , vegf receptors , biology , visual acuity , neuroscience
Purpose To explore the levels of vascular endothelial growth factor (VEGF) in vitreous and subretinal fluid in eyes with rhegmatogenous retinal detachment. Methods Overall 16 eyes of 16 patients (mean age, 49) with fresh rhegmatogenous retinal detachment were included into this study. There was no systemic or ocular disease other than the retinal detachment that might contribute to results in the patients. Vitreous samples ( n = 9) were obtained during pars plana vitrectomy whereas subretinal fluid samples ( n = 7) were obtained via external drainage during encircling sclera buckle surgery. Serum and vitreous VEGF levels were analyzed using enzyme‐linked immunosorbent assay. Total vitreous or subretinal fluid proteins and total serum proteins were also measured. Mann–Whitney U and Spearman correlation tests were performed to compare the VEGF concentrations. Results Median VEGF level was found higher in subretinal fluid when compared to vitreous (545.80 pg/ml vs. 106.08 pg/ml, p = 0.05). The difference was still significant after adjusting the results with total protein (p = 0.05). Subretinal fluid VEGF level was similar to serum VEGF level (410.30 pg/ml) in buckling group (p = 0.95) while serum VEGF level (221.20 pg/ml) was significantly higher than that of vitreous in vitrectomy group (p = 0.019). There was no correlation between serum and subretinal VEGF (p > 0.05) or serum and vitreous VEGF levels (p > 0.05). Conclusions The retinal pigment epithelium is the major source of VEGF in the eye. The current study confirmed that VEGF level in subretinal fluid is higher than that of in the vitreous in eyes with fresh retinal detachment, most probably due to the retinal pigment epithelium is in closer vicinity to the subretinal fluid, compared to vitreous humor. Acknowledgement This project was supported by Marmara University, Scientific Research Projects Committee.