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The predegenerated nerves extract enhances the endogenous neuroprotective system of Retinal Ganglion Cells by modulating of BDNF expression in rat glaucoma model
Author(s) -
PietruchaDutczak M.,
Smędowski A.,
LewinKowalik J.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0614
Subject(s) - endogeny , glaucoma , inner nuclear layer , neuroprotection , retinal , ganglion cell layer , ophthalmology , retinal ganglion cell , retina , immunohistochemistry , chemistry , endocrinology , medicine , biology , neuroscience
Purpose To verify impact of the administration of extract from predegenerated rat sciatic nerves on the expression and co‐localization of endogenous BDNF and whether this change promotes survival of RGCs in rat glaucoma model. Methods Experimental glaucoma was induced unilaterally (the right eye) based on modified bead model. The left eye served as control. Two, 7, or 14 days following glaucoma induction, nerve extract (PNE groups) or PBS was injected into the vitreous body. Time of exposure of RGCs to the extract was 2, 3, or 4 weeks. Five days before euthanasia, RGCs were labeled by FluoroGold (FG). Number of FG‐positive RGCs was estimated and expression of BDNF was analyzed in immunohistochemistry. Results BDNF was highly expressed in inner nuclear layer (INL) and RGC layer in the healthy eye and weakly in RGC layer in glaucoma. Injection of the extract at 14th day following induction of glaucoma and 3‐week‐exposure to the extract resulted in the strongest increase in endogenous BDNF expression in both INL and ONL and weaker in RGC layer what was correlated with the highest RGCs survival (2,943 ± 147 in PNE group vs. 2,212 ± 497 in PBS group; p < 0.05). BDNF expression in RGC layer co‐localized with RGCs markers and glial cells markers (both cells bodies and processes). Conclusions The increase in the endogenous BDNF expression is correlated with survival of RGCs and is the highest when the extract is administered 14 days following glaucoma induction acting for 3 weeks.