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Morphological, phisiological and immunocytochemical evaluation in patients with idiopathic epiretinal membranes
Author(s) -
Miniewicz J.,
Romanowska Dixon B.,
Petka O.,
Elas M.,
Sarna M.,
KubickaTrzaska A.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0558
Subject(s) - retinal , epiretinal membrane , medicine , ophthalmology , confocal , retina , retinal pigment epithelium , internal limiting membrane , macular hole , pathology , biomedical engineering , vitrectomy , optics , visual acuity , physics
Purpose To investigate changes of the vitreoretinal interface in patients with idiopathic epiretinal membranes (ERM). Methods Analysis of 23 patients treated by 25G PPV due to visual imparment caused by idiopathic ERM. BCVA and DRI OCT (Atlantis Topcon) imaging was performed before surgery and in one year follow up. Retinal and vitreoretinal interface morphology was evaluated and compared with changes in visual fuction. ERM and internal limiting membranes (ILM) were collected intraoperatively for immunocytochemical analysis. Photographeis of probes were made by scanning confocal microscope LSM 88‐ Zeiss, with seven laser lines, 32‐channel spectral detector GaAsP and two photomultiplier for fluorescence and one for transmission. Scanning module was connected with reverse optic microscope Axio Observer Z1 Zeiss with fluorecsent lamp and adequate filters. Results BCVA before surgery was from counting finger from 3 m to 0.5 (mean 0.1) and in one year of postoperative follow up ipmroved to 0.3–0.9 (mean 0.6). In 18 eyes (78%) vitreoretinal traction was visualized by OCT DRI before the surgery. Mean central retinal thickness (CRT) was 487 μ m. In patients with CRT more than 500 μ m macular oedema in preoprative scans was observed. In these cases the oedema was reduced in one year of postoperative observation. Microscopic and immunocytochemical analysis revealed a high amount of cells in ERM probes and low number of cells in ILM probes (immunoreactivity was positive for glial cells, retinal pigment epithelium, hyalocytes, actin and macrophages). Conclusions New techniques of visualisation and analysis improve possibilities of diagnostic and evaluation of treatment results in patients with ERM. These methods should be used as complementary. Evaluation of wider group of patients may improve the knowledge in the field of idiopathic ERM etiopathogenesis.