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In the search of biomarkers for thyroid associated orbitopathy (TAO)
Author(s) -
Kishazi E.,
Dor M.,
Eperon S.,
Gracià M.D.L.A.,
Fouda C.,
Oberic A.,
Hamédani M.,
Turck N.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0507
Subject(s) - tears , medicine , ingenuity , proteomics , disease , thyroid , pathway analysis , downregulation and upregulation , bioinformatics , immunology , biology , biochemistry , gene expression , neoclassical economics , economics , gene
Purpose Tears are known as lubricating the eyes and ensuring nutrition and protection of the surrounding ocular tissues. However, its composition is the result of a dynamic system, which is dependent on various stimuli, including ocular and systemic diseases. Here, we propose that, tears could represent an innovative source of biomarkers in thyroid‐associated orbitopathy (TAO) disease. Methods Schirmer's test was adopted to collect tears from TAO [ n = 20, 3 males, mean age (±SD): 46.0 years (±13.0)] and healthy patients [ n = 18, 8 males, 45.4 years (±18.7)]. Independent isobaric proteomics experiments were carried out and analyzed on a linear trap quadrupole Orbitrap Velos Pro. Easyprot software was used to obtain protein identification and quantification (2 unique peptides, 1% FDR, ratio<0.66 or >1.5, p‐value>0.05). Biological process and pathways were analyzed using Ingenuity pathway analysis software (6.7 version). Verification of proteins was performed by Western Blot or immunoassays. Results Globally, 646 proteins were identified and 62 were considered as differentially expressed (27 up‐and 35 downregulated). Interestingly, among them, the acute phase response signalling and glycolysis pathways were mainly represented. Verification is ongoing for these candidates. In parallel, we observed that the levels of IL‐6, IL‐10, IL‐12 and TNF‐ α , were significantly upregulated in tears of TAO patients. Conclusions These results confirmed tears as a suitable source to discover biomarkers for TAO disease. Moreover, the emergence of proteins involved in the glycolysis, associated to inflammation, could bring new general knowledge about TAO that still remains not well characterized. This study is kindly supported by the Provisu foundation and the SNF_MHV (PMPDP3_158370).