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Primary intraocular lymphoma and flow cytometry analysis of the vitreous – a case report
Author(s) -
Romanowska Dixon B.,
Karska Basta I.,
KubickaTrzaska A.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0482
Subject(s) - intraocular lymphoma , medicine , pars plana , uveitis , vitrectomy , intermediate uveitis , lymphoma , pathology , ophthalmology , population , visual acuity , anterior uveitis , environmental health
Purpose To present a case of chronic bilateral uveitis which preceded diagnosis of B‐cell intraocular lymphoma based on the results of a vitreous flow cytometry analysis. Methods We conducted a retrospective and interventional case report. Results A 46‐year‐old woman was admitted to the Department of Ophthalmology of Jagiellonian University in Krakow witha 6‐months history of deterioration of vision in both eyes. Clinical examination showed the presence of bilateral chronic uveitis with the presence of subretinal multiple yellow lesions. The standard screening panel laboratory tests for uveitis showed no pathology. MRI of a central nervous system was within normal limits. Because of no improvement in BCVA andthe presence of active intraocular inflammation inspite of long‐term systemic steroids and immunosuppressant therapy a diagnostic 25G pars plana vitrectomy was performed. A 1.5 cc of non‐diluted vitreous was taken and analyzed using a flow‐cytometry. The cytometric analysis of the vitreous aspirate showed the presence of 67.5% of lymphocyte B population (CD3+; CD2+, CD5+; CD7+, CD45RO+; CD38+, HLADR+/−; CD45RA−) with CD4/CD8 = 8.5. The diagnosis of B‐cell lymphoma was established. Conclusions Flow cytometry analysis of vitreous aspirate may be a precious diagnostic tool in unclear clinical cases of intraocular inflammation, in eyes suspected for intraocular neoplastic process.