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Comparison of several transport activities of lens epithelial cells from cataract and healthy dog
Author(s) -
Ochiai H.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0307
Subject(s) - transfection , lens (geology) , glutathione , ascorbic acid , glutamate receptor , cell culture , chemistry , medicine , biology , biochemistry , enzyme , genetics , food science , paleontology , receptor
Purpose The aim of this study is to compare the transport activities between normal and cataract lens epithelial cells. Methods The primary lens epithelial cells of healthy and cataract dog were transfected with the expression plasmid DNA of large T antigen from replication origin‐defective simian virus 40 (SV40), then cloned using a glass cylinder. The transport activities of glycine, cysteine, glutamate, arginine, leucine, carnitine, ascorbic acid and glutathione were investigated using two cell lines. Results There was no significant difference in transport activity between the two cells lines except for glutamate. Glutamate transport activity was 20% lower in cells from cataract than healthy one. Conclusions Reduced glutamate transport activity may contribute to the pathogenesis of canine cataract.