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Melissa officials L. extracts protect human retinal pigment epithelial cells against oxidative stress‐induced apoptosis.
Author(s) -
Kang S.,
Shin J.A.,
Oh J.,
Rho C.R.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0253
Subject(s) - oxidative stress , apoptosis , reactive oxygen species , poly adp ribose polymerase , protein kinase b , microbiology and biotechnology , chemistry , melissa officinalis , pi3k/akt/mtor pathway , intracellular , biology , biochemistry , polymerase , enzyme , botany
Purpose We evaluated the protective effect of ALS‐L1023, an extract of Melissa officinalis L. (Labiatae; lemon balm) against oxidative stress‐induced apoptosis in human retinal pigment epithelial cells (ARPE‐19 cells). Methods ARPE‐19 cells were incubated with ALS‐L1023 for 24 h and then treated with hydrogen peroxide (H2O2). Oxidative stress‐induced apoptosis and intracellular generation of reactive oxygen species (ROS) were assessed by flow cytometry. Caspase‐3/7 activation and cleaved poly ADP‐ribose polymerase (PARP) were measured to investigate the protective role of ALS‐L1023 against apoptosis. The protective effect of ALS‐L1023 against oxidative stress through activation of the phosphatidylinositol 3‐kinase/protein kinase B (PI3K/Akt) was evaluated by Western blot analysis. Results ALS‐L1023 clearly reduced H2O2‐induced cell apoptosis and intracellular production of ROS. H2O2‐induced oxidative stress increased caspase‐3/7 activity and apoptotic PARP cleavage, which were significantly inhibited by ALS‐L1023. Activation of the PI3K/Akt pathway was associated with the protective effect of ALS‐L1023 on ARPE‐19 cells. Conclusions ALS‐L1023 protected human RPE cells against oxidative damage. This suggests that ALS‐L1023 has therapeutic potential for the prevention of dry age‐related macular degeneration.