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Investigating the effect of low dose ionising radiation on epithelial progenitor cell niches
Author(s) -
Quinlan R.,
Kalligeraki A.,
Pal R.,
Wu J.J.,
Inagaki M.,
Tanaka H.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0239
Subject(s) - epithelium , lens (geology) , confocal , ionizing radiation , hormesis , confocal microscopy , microbiology and biotechnology , progenitor cell , cell , biology , pathology , irradiation , stem cell , medicine , optics , endocrinology , physics , biochemistry , paleontology , oxidative stress , nuclear physics
Summary Recent work has identified a nonlinear biological response to low dose radiation in support of the low dose hormesis model in the murine lens epithelium, with mice being irradiated with doses between 0.1 and 1 Gy, with the dose response peaking at 0.5 Gy (Markiewicz et al., 2015). An important question is whether other epithelia show similar low dose hormetic responses and whether there are any associated pathologies. These comparative studies will help us understand how low dose IR causes cataract. We have developed a conical gel mount system on glass microscopy slides for the mouse lens, which allows its placement and manipulation using a standard inverted confocal microscope. Each lens was subsequently imaged with the Leica SP5 confocal microscope through a set of sequential z stacks, and reconstructed in ImageJ to produce the 3D anaglyphs to measure cell density, cell proliferation and cell apoptosis events in the lens epithelium. By comparison, conventional histochemistry was sufficient to make similar measurements for the progenitor cell niches in the hair follicle and colonic crypts and therefore to determine whether there is a common or distinct hermetic response by the lens epithelium to low dose ionizing radiation.