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Retina proteomics provide new insights in glaucoma
Author(s) -
Funke S.,
Perumal N.,
Schmelter C.,
Teister J.,
Markowitsch S.,
Beck S.,
Pfeiffer N.,
Grus F.H.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0128
Subject(s) - retina , glaucoma , proteome , optic nerve , proteomics , retinal , quantitative proteomics , biology , ophthalmology , medicine , chemistry , bioinformatics , gene , neuroscience , biochemistry
Summary Proteomic alterations have been studied in retina samples of glaucoma and non‐glaucoma control donor eyes ( N = 5/group) by use of a bottom up proteomic platform implementing LC ESI LTQ Orbitrap XL MS analysis and label‐free quantification following functional analysis using gene ontology (GO) annotation. Furthermore, candidate abundances were examined in porcine retina and optic nerve head preparations ( N = 12/tissue type). Approximately 10% of identified proteins (>600, FDR < 1%) showed significant level alteration (p < 0.05) or distinct tendencies in glaucomatous retinae, predominantly encircling mitochondrial and nucleus residing proteins. Thereby, new candidates, e.g. ADP/ATP translocase 3 and methyl‐CpG‐binding protein 2 could be proposed to be associated to glaucoma. Numerous candidates, e.g. retinol‐binding protein 3 show characteristic distribution of abundance comparing optic nerve head and retina proteomes in porcine model eyes (p < 0.05). In conclusion, distinct proteomic alterations could be documented in the human glaucomatous retina highlighting new retinal protein candidates. Moreover, characteristic distribution of ocular proteins and candidates could be revealed.