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Counting retinal neurons in the adult rat retina
Author(s) -
VidalSanz M.,
JiménezLópez M.,
NadalNicolás F.M.,
OrtínMartínez A.,
ValienteSoriano F.J.,
Rovere G.,
SalinasNavarro M.,
AvilésTrigueros M.,
AgudoBarriuso M.,
VillegasPérez M.P.
Publication year - 2016
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2016.0039
Subject(s) - retina , dapi , ganglion cell layer , calretinin , biology , retinal , optic nerve , inner nuclear layer , ganglion , ocular hypertension , anatomy , giant retinal ganglion cells , microbiology and biotechnology , retinal ganglion cell , staining , neuroscience , immunohistochemistry , immunology , biochemistry , glaucoma , genetics
Summary We study in adult albino rats long‐term effects on the ganglion cell and photoreceptor layers of the retina after ocular hypertension (OHT) or introarbital optic nerve transection (IONT). OHT was induced by lasering limbar tissues while IONT was performed at 0.5 mm from the optic disc (OD). The ganglion cell layer (GCL) was investigated in wholemounts to identify, count and map RGCs (identified with Fluorogold labelling, or Brn3a or melanopsin (m) antibodies), cells in the GCL (DAPI‐staining to detect nuclei in this layer or calretintin antibodies to identify displaced amacrine cells). The distribution of S‐ and L‐cones was studied in wholemounts immunoreacted for S‐ and L‐opsin. Following OHT there were significant diminutions of orthotopic and displaced Brn3a + RGCs in pie‐shaped sectors. These sectors had large numbers of DAPI + nuclei, calretinin + cells and m + RGCs. The S‐ and L‐cone populations diminished to 65 and 80%, or to 20 and 35% at 1 or 6 months, respectively, indicating severe cone loss. IONT resulted at 15 days in massive loss of Brn3a + RGCs or m + RGCs throughout the retina and at 15 months the loss represented <1 or 35% of the original populations, respectively, indicating a greater survival for m + RGCs.