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Combination of Resveratrol with omega‐3 fatty acids synergize to counteract VEGF ‐R pathway in sick retinal pigment epithelium cells mimicking AMD
Author(s) -
Chalons P.,
Scagliarini A.,
Courtaut F.,
Olmière C.,
Delmas D.
Publication year - 2015
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2015.0604
Subject(s) - macular degeneration , retinal , resveratrol , blot , retinal pigment epithelium , signal transduction , vascular endothelial growth factor , cancer research , chemistry , medicine , microbiology and biotechnology , vegf receptors , biology , pharmacology , biochemistry , ophthalmology , gene
Purpose The aim of this work is to assess whether RSV (Resveratrol) can act synergistically with omega‐3 fatty acids to modulate VEGF (Vascular Endothelial Growth Factor) signaling pathway in order to identify a new and more effective therapy for the treatment of AMD (Age‐Related Macular Degeneration). Methods In this study, undifferentiated and differentiated human retinal pigment epithelial cells ( ARPE ‐19) were used. The cells were treated with an omega‐3/ RSV preparation (Resvega ® ), or a RSV ‐free formulation or RSV alone for 24 h. The expression of key proteins in VEGF signaling pathway was evaluated by Western Blotting. Results We observed that the combination omega‐3/ RSV preparation (Resvega ® ) induces a stronger inhibition of the VEGF ‐R pathway activation than the RSV ‐free formulation or RSV alone in sick retinal cells. Surprisingly, Resvega ® maintains and increases the functional VEGF ‐R pathway in normal retinal cells. Conclusions This work brings a new insight into the mechanism by which omega‐3/ RSV could counteract AMD and could protect the other eye when AMD is already present. Financial Support: This work is supported by Théa laboratory.