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Electrophysiological ON and OFF responses in Autosomal Dominant Optic Atrophy
Author(s) -
Morny E.,
Margrain T.,
Binns A.,
Votruba M.
Publication year - 2015
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2015.0504
Subject(s) - erg , photopic vision , electroretinography , ophthalmology , optics , amplitude , flash (photography) , physics , medicine , retina
Purpose To assess the effect of ADOA on the ON and OFF components of the photopic negative response (Ph NR ). Methods Twelve participants from 6 families with OPA 1 ADOA and 16 age matched controls were recruited. Electrophysiological assessment involved long flash focal (20o) and full field ERG s using red flash (664 nm, 250 msec, 55 cd/m 2 , 2 Hz) on a rod saturating blue background (454 nm, 100 scot cd/m 2 ); and brief xenon flash ERG s using red filter (Lee Filter “Terry Red”, max 300 µs flash duration, 1.69 cd.s.m‐2, 4 Hz) over a continuous rod saturating blue background (Schott Glass Filter BG 28, 206 scot cd/m 2 ). Amplitudes (from peak and baseline to fixed time point) and implicit times of the ERG components were analysed. Results Mean amplitude (peak to fixed time) of the focal Ph NR ‐ ON were significantly (p < 0.05) reduced by 40% while the focal Ph NR ‐ OFF was completely eliminated. In the long duration full field ERG , the Ph NR ‐ ON and – OFF were reduced by 21% and 57% respectively. Subtraction of the grand averaged ERG of ADOA participants from that of the controls produced a difference plot with a nearly symmetrical loss in the Ph NR ‐ ON and OFF components of the focal ERG . ROC curve analysis showed focal Ph NR ‐ ON and OFF amplitudes performed better than their full field counterparts. Conclusions We show that OFF components of the photopic ERG were more severely affected in ADOA than ON components. Additionally, the focal Ph NR ‐ ON and – OFF components were more effective in assessing ADOA than their full field components.