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cis‐Urocanic acid prevents inflammation and cell death in UVB ‐treated ARPE ‐19 cells
Author(s) -
Korhonen E.,
Piippo N.,
Hytti M.,
Kaarniranta K.,
Kauppinen A.
Publication year - 2015
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2015.0427
Subject(s) - lactate dehydrogenase , urocanic acid , viability assay , secretion , chemistry , cell , inflammation , microbiology and biotechnology , epidermis (zoology) , enzyme , biochemistry , biology , immunology , anatomy , histidine
Purpose cis‐Urocanic acid (cis‐ UCA ) is an endogenous ultraviolet ( UV ) absorbing chromophore that is mainly produced in the upper layers of epidermis. The aim of our study was to investigate the cytoprotective capacity of cis‐ UCA in UVB ‐irradiated ARPE ‐19 cells. Methods ARPE ‐19 cells were pretreated with IL ‐1 α ± and cis‐ UCA and then exposed to UVB radiation. Secretion of IL ‐1 β and ‐18 was measured using the ELISA method. Moreover, the cells were observed under an inverted microscope and cell viability was measured by lactate dehydrogenase ( LDH ) release assay. The proper cis‐ UCA concentration was evaluated comprehensively beforehand using MTT (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide), neutral red, and LDH tests. Results Our results show that UVB after IL ‐1 α ± priming activates IL ‐1 β and ‐18 secretion in ARPE ‐19 cells and cis‐ UCA clearly alleviates that. cis‐ UCA also improves cellular viability. Conclusions cis‐ UCA shows anti‐inflammatory and cytoprotective properties in ARPE ‐19 cells.