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Role of PACAP for impairment of cornea epithelial wound healing
Author(s) -
SEKI T,
NAKAMACHI T,
FARKAS J,
WATANABE J,
IZUMI S,
SHIODA S
Publication year - 2014
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2014.s058.x
Subject(s) - cornea , corneal epithelium , immunostaining , wound healing , ophthalmology , epithelium , medicine , saline , contact lens , receptor , immunohistochemistry , pathology , surgery
Purpose Corneal epithelium disorder causes by infection, chemical burn, use of contact lens and eye drops like benzalkonium chrolide. Pituitary adenylate cyclase‐activating peptide, PACAP is known to have trophic effects on various tissues. PACAP also exists in eye tissues, however, the effects on corneal epithelial wound healing is still unknown. The aim of this study is to investigate the therapeutical effects of PACAP on wound healing of mouse cornea. Methods Cornea injury was performed in adult C57BL/6 male mice. One mm in diameter wounds were created on the cornea with a biopsy punch and the epithelium was removed. PACAP or saline were dropped on the eyes in every two hours until 12 hours post injury. Photographs were taken 0 h, 12 h and 24 h post injury with fluorescein staining. We also examined immunostaining or RT‐PCR against PAC1 receptor in the corneal tissue. Results We found that the area and perimeter of corneal injury were significantly decreased in PACAP‐treated group compared with saline‐treated group. In this time, the most effective does was 10‐11M. The expression of PAC1 receptor was detected in the corneal epithelium with RT‐PCR and immunostaining. PACAP‐induced corneal healing was inhibited by co‐administration of PAC1 receptor antagonist, PACAP 6‐38. Conclusion These results suggest that PACAP stimulates the corneal wound healing via PAC1 receptor.