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PGE2 and PGF2a stimulation of porcine retinal arterioles in vitro is associated with Ca2+ activity in retinal perivascular cells
Author(s) -
KUDRYAVTSEVA O,
BEK T
Publication year - 2014
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2014.4744.x
Subject(s) - retinal , myograph , ryanodine receptor , chemistry , stimulation , contraction (grammar) , retina , pericyte , medicine , endoplasmic reticulum , biology , in vitro , neuroscience , biochemistry , endothelial stem cell
Purpose Disturbances in the regulation of retinal blood flow are a hallmark of vision threatening diseases. Retinal blood flow is controlled by autoregulation, which involves Ca2+ activity in a recently identified population of perivascular cells with pericyte characteristics (PVCs). The purpose of the study was to identify the sources of Ca2+ recruitment in these PVCs during stimulation of porcine retinal arterioles in vitro with PGE2 and PGF2a. Methods Porcine retinal arterioles with preserved perivascular retinal tissue were mounted in a confocal myograph, placed in a confocal microscope and loaded with Oregon Green. After addition of PGE2 (10‐5 M) or PGF2a (10‐5 M) arteriolar tone and fluorescence from PVCs were recorded simultaneously in the absence and in the presence of Ca2+ channel blockers (ryanodine, nifedipine, LOE908, CPA, 2‐APB). Results PGE2 induced significant relaxation of preconstricted retinal arterioles and Ca2+ activity in PVCs. The percentage of active PVCs, and the number but not the amplitude of Ca2+ oscillations were significantly reduced by CPA and 2‐APB, but not by ryanodine, nifedipine and LOE908. PGF2acaused arteriolar contraction and the percentage of active PVCs, the number and the amplitude of Ca2+ oscillations were significantly reduced by CPA and 2‐APB. Conclusion Ca2+ activity in PVCs accompanies porcine retinal arterioles responses to PGE2 and PGF2aSarcoplasmic reticulum and inositol triphosphate receptors are important sources of this Ca2+ activity. The amplitude of Ca2+ oscillations seems to play a particular role in contraction of retinal arterioles.