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Human limbal epithelial cells cultured on dense fibrillated collagen I transparent matrices
Author(s) -
BORDERIE V,
GHOUBAY D,
MOSSER G
Publication year - 2014
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2014.4734.x
Subject(s) - stroma , epithelium , microbiology and biotechnology , chemistry , scaffold , materials science , pathology , biomedical engineering , biology , medicine , immunohistochemistry
Transparent (78 to 84% at 580nm) collagen I fibrillated matrices have been synthesized at different concentrations making use of liquid crystal properties of collagen. The 90 mg/mL scaffold presents an organization that resembles the ones found in corneal stroma. Although matrices present low mechanical properties (with Young’s modulus of 2kPa at most) they allow development of an epithelium presenting the main characteristics of the corneal epithelial cells (expression of cytokertin 3 and presence of desmosomes) and maintenance of stemness during culture (i.e. expression of Delta N p63α and formation of holoclones in colony formation assay). Matrices with cultured cells feature higher transparency compared with matrices with no cells.