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Brillouin spectroscopy and lens refilling
Author(s) -
STACHS O
Publication year - 2014
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2014.4652.x
Subject(s) - brillouin spectroscopy , optics , lens (geology) , materials science , confocal , brillouin scattering , brillouin zone , rheology , refractive index , spectroscopy , confocal microscopy , optical fiber , physics , composite material , quantum mechanics
The confocal Brillouin spectroscopy is an innovative measurement method that allows the non‐invasive determination of the rheological properties of materials. Its application in ophthalmology can offer the possibility to determine in‐vivo the biomechanical properties of transparent biological tissue such as the crystalline lens. This seems to be a promising approach concerning current presbyopia research. Due to the spatially resolved detection of the viscoelastic lens properties, a better understanding of the natural aging process of the lens and the influences of different lens opacities on the stiffness is expected. From the obtained spectral data the relative protein levels, the relative refractive index profile and the relative density profile within the lens tissue can be derived. A measurement set‐up for confocal Brillouin microscopy based on spectral analysis of spontaneous Brillouin scattering signals by using a high‐resolution dispersive device is presented. In‐vitro and in‐vivo test results on native and artificial lenses are presented and evaluated concerning their rheological significance.