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Can we trust the biopsy for prognostication in patients with choroidal malignant melanomas?
Author(s) -
KIILGAARD J,
BAGGER M,
TOLSTRUP ANDERSEN M,
HEEGAARD S,
KLARSKOV ANDERSEN M
Publication year - 2014
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2014.4245.x
Subject(s) - biopsy , multiplex ligation dependent probe amplification , medicine , melanoma , choroidal melanoma , pathology , fluorescence in situ hybridization , radiology , chromosome , biology , cancer research , gene , biochemistry , exon
Purpose The purpose was to validate the minimal invasive TransVitreal‐RetinoChoridal (TVRC) biopsy as prognostic tool for uveal melanoma. Methods Thirty‐six patients treated for choroidal melanoma at Rigshospitalet, Copenhagen from 1st January 2009 to 31st December 2011 were included. All patients had a minimal invasive TVRC biopsy performed to confirm the diagnosis before the eye was enucleated. Fluorescence in situ hybridization (FISH) for chromosome 1, 3, 6 and 8 as well as multiplex ligation‐dependent probe amplification (MLPA) were performed on all biopsy samples. Paraffin embedded sections of the enucleated eye were then divided into 3 mm hexagons. Each hexagon were then individually analyzed with MLPA in order to elucidate eventual heterogeneity of chromosomal status of the tumor. The MLPA results from the tumor were then compared to the results from the biopsy. Results This is an ongoing study and so far results from 6 eyes have been obtained. All tumors showed heterogeneity of their chromosomal status. In 5 of 6 tumors more chromosomal abnormalities were found in the vicinity of the optic nerve compared to the periphery. In 4 of 6 tumors there was a complete correspondence between biopsy result and worst chromosomal status of the tumor. The remaining 2 biopsies underestimated the chromosomal changes, but it did not affect the prognostic value. Conclusion Minimal invasive TVRC biopsy technique for choroidal melanoma seems to be useful for prognostic purposes.