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Pinosylvin protects retinal pigment epithelial cells from oxidative stress by activating Nrf2‐mediated antioxidant defence system
Author(s) -
KOSKELA A,
REINISALO M,
HYTTINEN J,
KAARNIRANTA K,
KARJALAINEN R
Publication year - 2014
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2014.3255.x
Subject(s) - oxidative stress , viability assay , cytoprotection , chemistry , glutathione , antioxidant , oxidative phosphorylation , retinal , mtt assay , lipid peroxidation , heme oxygenase , hmox1 , microbiology and biotechnology , cell , biochemistry , heme , biology , enzyme
Purpose The constant light exposure, high metabolic rate and high lipid concentration expose retinal pigment epithelial (RPE) cells to chronic oxidative stress, which may lead to RPE cell degeneration and evokes secondarily photoreceptor damage and visual loss. In this work, we assessed the protective role of wood derived polyphenol, pinosylvin (PS), in the prevention of oxidative stress and the molecular mechanisms behind these effects Methods ARPE‐19 cells were exposed either to PS or hydroquinone (HQ) or both simultaneoulsy. Toxicity and protective effects of PS against HQ‐induced oxidative stress were determined by colorimetric cell viability test (MTT‐test). Signalling mechanism of PS was studied by analyzing mRNA levels of Nrf2 (nuclear factor‐erythroid 2‐related factor‐2) and its target genes heme oxygenase‐1 (HO‐1), glutathione S‐transferase pi 1 (GSTP1) and sequestosome 1 (p62) by PCR. Moreover, Nrf2 and p62 were silenced by using siRNA technology. Results PS treatment protected ARPE‐19 cells from HQ, when analyzed by MTT assay. PS treatment significantly increased the expression of HO‐1 that was abolished by Nrf2 siRNA . PS did not protect p62 siRNA‐treated cells from HQ induced oxidative stress. Conclusion Our results suggest that PS treatment conferred protection against HQ‐induced oxidative stress through the induction of HO‐1 in ARPE‐19 cells. Consequently, PS‐stilbene compounds may possess health‐promoting properties against oxidative stress‐related diseases such as AMD.