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miRNA and conjunctival melanoma migration
Author(s) -
MOULIN A,
NICOLAS M
Publication year - 2013
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2013.3746.x
Subject(s) - melanoma , downregulation and upregulation , cell culture , cell migration , in vivo , cancer research , cell , medicine , in vitro , pathology , biology , biochemistry , genetics , microbiology and biotechnology , gene
Purpose : In cutaneous melanoma, downregulation of miR‐211 has been implicated in migration and invasion. As we have previously demonstrated the downregulation of miR‐211 in conjunctival melanoma in vivo, we evaluated in vitro the role of miR‐211 in the migration of 4 conjunctival melanoma cell lines compared to a cutaneous melanoma cell line. Methods : Migration assays were performed using a cutaneous melanoma cell line and 4 conjunctival melanoma cell lines. Taqman RT‐PCR was performed to evaluate the level of miR‐211 in each cell line. Results : Compared to the cutaneous melanoma cell line with a high migration potential, 2 conjunctival melanoma cell lines demonstrated moderate migration abilities while 2 others no migration. The migration potential of the cell lines was significantly inversely correlated with Taqman evaluation of miR‐211 expression (p=0,0239). Conclusions : The migration ability of conjunctival melanoma cell lines is lower than a cutaneous melanoma cell line. The level of miR‐211 expression in conjunctival melanoma appears in vitro to inversely correlate with the migration potential of the cell lines, confirming the downregulation of miR‐211 observed in vivo in conjunctival melanoma.