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Long‐term nanostructural effects of high radiofrequency treatment on the rabbit skin tissues
Author(s) -
JAE HO S
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.t027.x
Subject(s) - collagen fibril , fibril , histology , in vivo , rabbit (cipher) , chemistry , medicine , pathology , anatomy , biology , biochemistry , statistics , microbiology and biotechnology , mathematics
Purpose The purpose of this study was to investigate the long‐term effects of high radiofrequency (HRF) tissue‐tightening treatment in in vivo rabbit dermal collagen fibrils. Methods These effects would be measured at different energy and at varying pass procedures on the nanostructural response level using histology and AFM analysis. Each rabbit was into one of seven experimental groups, which included the following: control group, and six HRF group according to three HRF energy (10 W, 20 W and 30 W) and two HRF pass procedures. The progressive changes in the diameter and D‐periodicity of rabbit dermal collagen fibrils were investigated in detail over one month post‐treatment period. Results Normal dermal tissues show an irregular collagen network structure, whereas those treated with the HRF tissue‐tightening device showed more prominent inflammatory responses with inflammatory cell ingrowth compared to the control as well as a well‐aligned collagen network structure at the nanostructural level. This effect showed more prominent with the passage of day after treatment. Although an increase in the diameter and D‐periodicity of dermal collagen fibrils was identified immediately after the HRF treatment, a decrease in the morphology of dermal collagen fibrils continued until the proliferative period. The dimensions of collagen fibrils returned to level of the control group at the postoperative 7 days. Conclusion A multiple pass treatment at low energy rather than a single pass treatment at high energy showed the changes in the collagen fibrils at the nanostructural level.