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Experimental study of the effect of femtosecond laser radiation on eye fundus and sclera morphology
Author(s) -
ZADOROZHNYY O,
PASYECHNIKOVA N,
KOROL A
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.s079.x
Subject(s) - sclera , laser , materials science , femtosecond , optics , retinal pigment epithelium , retinal , fundus (uterus) , retina , ophthalmology , medicine , physics
Purpose To study the macro‐ and microscopic changes in the structures of fundus and sclera in the experiment after exposure of femtosecond laser radiation with a wavelength of 800 nm. Methods As a source of laser radiation we used Ti: sapphire laser oscillator «Coherent», which allows to obtain a sequence of pulses of duration 130 fs and a repetition frequency of 76 MHz (wavelength ‐ 800 nm) and femtosecond regenerative amplifier Legend F‐1K‐HE, which provides a pulse frequency of 1 kHz, lasting no more than 130 fs and an energy of about 2.5 mJ. In th study of eye tissues after femtosecond laser exposure was carried out on enucleated porcine and bovine eye Results After exposure of the laser radiation with 76 MHz frequency were revealed destruction of the photoreceptor layer, the layer of retinal pigment epithelium and endothelium of blood vessels in the retina. Observed the effect of coagulation. When using the laser frequency of 1 kHz are marked changes in the fundus structures associated only with the cavitation effect of the laser. After the femtosecond laser damage to the sclera have been reported the formation of a defect of the sclera, full thickness homogenization of the collagen fibers of the sclera, the disappearance of cells, destruction of pigment cells of uveal tract and retinal pigment epithelium. Conclusion During 130 fs laser using with 800 nm wavelength and high pulse frequency (76 MHz) marked cavitation effect in the tissues and thermal damage of retinal structures, mainly photoreceptor layer and retinal pigment epithelium. After laser radiation with low frequency (1 kHz) using dominates the mechanical cavitation effects on retinal and scleral structures.