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Digital analysis of the changes in carcinomatous conjunctiva using Aperio nuclear v9 algorithm
Author(s) -
ASOKLIS R,
PAULAVICIENE R,
PETROSKA D
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.s002.x
Subject(s) - conjunctiva , medicine , pathology , immunohistochemistry , staining , h&e stain
Purpose To analyze and evaluate biological and morphometrical changes and differences of healthy and carcinomatous conjunctiva using Aperio nuclear v9 algorithm. Methods A pilot study for the objective evaluation of carcinomatous conjunctiva in 6 cases. There were analyzed 6 excised conjunctival tumors specimens from 6 patients (6 eyes). Medium age of the patients was 68 years. There were two males and four females. In 3 patients (3 eyes) conjunctival tumor was excised, cryoaplication and amniotic membrane transplantation was performed. In 3 patients excisional biopsy was performed without additional surgical manipulations. Conjunctival specimens were stained with hematoxilin and eosin. Immunohistochemical (IHC) staining with a proliferation marker of cells Ki‐67 was performed for all specimens. Digital slides of the IHC of Ki‐67 from these biopsies were obtained by Aperio ScanScope GL. Aperio Nuclear V9 algorithm was applied to assay proliferation indices (PI) of nuclei stained with Ki‐67 and size of all nuclei in both abnormal and normal conjunctiva. Results There were analyzed from 1585 till 5020 malignant and 242‐714 normal cells. PI of Ki‐67 ≥19% (range 19.3‐62.1%) was found in carcinomatous conjunctiva comparing with normal conjunctiva PI ≤13.5% (range 1.9‐21.6%) in the same patients. No significant difference was found between nucleus size in pathological (mean 41 µm2, range 33‐64 µm2) and normal conjunctiva (mean 35.5 µm2, range 34‐36 µm2). Conclusion Aperio nuclear algorithm can be used for carcinomatous and healthy conjunctiva analysis. Further studies of the malignant and healthy eye tissues using larger cohort are required.