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Fine needle aspiration biopsy in uveal melanoma : the Curie experience
Author(s) -
DESJARDINS L,
LEVY C,
LUMBROSOLEROUIC L,
CASSOUX N,
SASTRE X,
ASSELAIN B,
PLANCHER C,
COUTURIER J
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.4467.x
Subject(s) - medicine , biopsy , melanoma , fine needle aspiration , radiology , surgery , cancer research
Purpose In order to posssibly include uveal melanoma patients in a randomized trial on adjuvant therapy by Fotemustine we have started in 2009 to propose fine needle aspiration biopsy for genetic studies during clip or plaque positioning Methods Biopsies were proposed to patients with uveal melanomas of more than 5 mm in thickness.we have used transcleral or transvitreal biopsies depending on the location of the tumor with 25 or 23 gauge needles. Histoacryl glue was used after transcleral biopsies and cryotherapy for transvitreal biopsies.Patients signed an inform consent Results 108 biopsies were performed between January 2009 and april 2012; 6 patients had transient vitreal hemorrhages ( much more frequent with transvitreal p<0,0001))but no other side effects was observed. A sufficient quantity of DNA for CGH array was present in76% of cases, using whole genome amplification in 60% of cases. The quality of the specimen was strongly correlated with the presence of tumor cells’ p<0, 0001). 28, 7% of patients were classified as high genomic risk. No correlation was found between location and size of the tumor or any other clinical factor and the biopsy success rate Conclusion fine needle aspiration biopsy is a safe procedure to allow genetic testing in uveal melanoma patients