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Adjuvant stem cell‐based therapy in acute retinal injury after sodium iodate administration in mice
Author(s) -
MACHALINSKA A
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.4286.x
Subject(s) - transplantation , retinal , retina , medicine , electroretinography , regeneration (biology) , retinal degeneration , stem cell , pathology , ophthalmology , neuroscience , biology , microbiology and biotechnology
Purpose The aim of this study was to determine and optimize a new strategy of SC‐based therapy of selectively damaged retina after sodium iodate (NaIO3) administration in C57BL/6J mice. Methods To address this issue, we investigated the effects of NaIO3 administrated in two different concentrations, i.e. 40 and 20 mg per kg of the body mass. Electrophysiological function of the retina using dark‐and light‐adapted full field flash ERG as well as morphological characteristics, were determined at several time points after each dose administration. Next, we performed intravitreal transplantation of murine GFP+Lin‐ cells on the 1st day since NaIO3 administration. We analyzed the retinal functional changes as well as the number, localization and phenotype of intravitreally injected GFP+Lin‐ cells within recipients’ retinas. Results Our findings revealed that massive destruction of the tissue was associated with irreversible retinal dysfunction, whereas moderate retinal injury triggered regenerative mechanisms that restore bioelectrical function of the damaged retina.By employing SC‐based therapy we achieved noticeable improvement of the retinal function, particularly in the short‐term observation. We observed the presence and proliferation of the injected cells at the site of RPE injury. Conclusion Our study provides evidence that NaIO3‐induced retinal damage triggers a sequence of pathophysiological events dedicated to supporting the self‐regeneration of injured tissue. Our results indicate that if the scope of retinal destruction is profound, endogenous regeneration is ineffective and may ultimately require therapeutic transplantation of specific stem cell subpopulations and other adjuvant therapies.