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Experimental models of intraocular inflammation
Author(s) -
BEUERMAN R,
YUAN ZH
Publication year - 2012
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2012.1623.x
Subject(s) - tropicamide , medicine , sodium hyaluronate , ophthalmology , cataract surgery , phacoemulsification , cornea , surgery , intraocular lens , pupil , visual acuity , neuroscience , biology
Purpose Intra‐ocular surgery is usually followed in the near term post‐operative perios by a course of steroids or NSAIDS. However, the nature of post‐surgical inflammation is not well known due to the problems with developing an useful model. This talk describes the issues and outcomes using a mouse model of cataract surgery. Methods Cataract surgery was simulated by an approach 0.4mm posterior ttp to the limbus using a sapphire knife. Pupillary dilation was achieved by using 1% tropicamide and 2.5% phenylephrine. A corneal incision, of ∼30–60°, was made, and 1% sodium hyaluronate was inserted into the anterior chamber. The corneal incision was then extended to ∼90°, and an anterior curvilinear continuous capsulorrhexis was performed and lens was removed by forceps. The anterior chamber was filled with 1% sodium hyaluronate, and the corneal wound was closed using interrupted sutures. Topical 2.5% phenylephrine, 1% tropicamide, and 1% atropine were administered at the end of the surgery. Antibiotics were administered after surgery three times/day. Results In vivo confocal microscopic analysis showed cell infiltration was not observed in the anterior chamber in the control eye, but seen in the wounded eye at PO day 1(66±5 cells). Cells in the anterior chamber peaked at day 3 (316±52 cells), and decreased over time to day 7 (3±1 cells). RT‐PCR showed that S100A8/9 mRNA levels in the iris were up‐regulated significantly (p<0.05) at PO day 1, peaked at day 3, and remained elevated to day 7. mRNA levels of S100A8/9 in the cornea, HMGB1 in the cornea and iris increased significantly (p<0.05) at PO day 1 and decreased over time. Immunostaining for S100A8/9 and HMGB1 corroborated the RT‐PCR results. There was no detectable S100A8/9 or HMGB1 in the unwounded iris. The expression of S100A8/9 and HMGB1 was stimulated in the cornea stroma and iris, with a peak at PO day 3, and decreasing over time. Conclusion A mouse model and potentially other animal models of cataract surgery could be useful to develop new methods for control of intra‐ocular inflammation.