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The Sanger Mouse Genetics Programme: High Throughput Characterisation of Knockout Mice
Author(s) -
GARDIN A,
WHITE J
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.4451.x
Subject(s) - sanger sequencing , phenotype , mutant , knockout mouse , computational biology , biology , genetics , gene , gene knockin , mutation
Purpose The Sanger Mouse Genetics Project (MGP) is committed to making a significant contribution to the functional annotation of the mammalian genome by generating, characterising and archiving in the order of 200 lines of knockout mice per year, including 125 lines which have been processed as part of the EUMODIC consortium. Phenotypic data on a spectrum of disease conditions are obtained for each mouse line without the need for any prior assumptions about function by performing a standardised battery of phenotyping screens. The data generated will help to further the understanding of the interplay of genes and disease and provide an insight into the various underlying biological pathways. All phenotyping data and biological resources generated by the project are openly available to the scientific community. Methods Eye morphology is routinely assessed using a standard parameter assessment list in conjunction with the Slit Lamp and Ophthalmoscope and images are collected when abnormalities are identified. Expression profiling via the lacZ reporter gene is performed for each mutant line in adults and at E14.5. We collaborate with University of Iowa who are performing a pathology review of H&E stained paraffin sections and also sectioning of lacZ positive eyes. Results To date, the eye screen has been completed on over 310 mutant lines. Here we report examples of novel eye‐related abnormalities identified by the eye morphology, embryonic lethality and/or expression screens performed by the Sanger MGP. We will present how to identify a potentially interesting mouse mutant on our database and discuss the impact our knockout mouse models might have on your research.