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Increased IL‐6 levels in the vitreous of proliferative diabetic retinopathy (PDR)
Author(s) -
ARJAMAA O,
PÖLLÖNEN M
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.353.x
Subject(s) - diabetic retinopathy , medicine , neovascularization , interleukin 8 , retinopathy , analysis of variance , mann–whitney u test , diabetes mellitus , ophthalmology , inflammation , endocrinology , angiogenesis
Purpose To assess the activity of HIF‐1alpha and NF‐kappaB transcription factors and inflammation markers (IL‐6 and IL‐8) in the vitreous of patients suffering from proliferative diabetic retinopathy. Methods Twenty seven patients were enrolled for the study. The severity of retinopathy was classified (0, 1, 2, 3, 4) and the activity of neovascularization was graded (0, 1, 2, 3, 4) intraoperatively. Samples were collected during surgery and the NF‐κB and HIF‐1alpha and transcription factors activity and expression levels of IL‐6 and IL‐8 were measured. Results The majority of samples fell into the retinopathy classes 3 (n=12) or 4 (n=13). Neither the level of IL‐6 increased from 68.9 + 46.8 pg/ml to 102.7 + 94.1 pg/ml and IL‐8 from 165.1 + 136.0 pg/ml to 521.0 + 870.9 pg/ml (mean + SD, non‐significant change: normality test followed with Mann‐Whitney Rank Sum Test). According to the neovascularization activity the samples fell into the grades 1 (n=7), 2 (n=12) or 3 (n=7). In IL‐6 there was a statistically significant increase (p<0.05) from grade 2 to 3: 58.6 + 40.3 pg/ml and 158.4 + 102.5 pg/ml, respectively (Kruskal‐Wallis One Way ANOVA on Ranks followed with Dunn’s Method). The level of IL‐8 was in grade 1: 118.0 + 62,4 pg/ml, in grade 2: 192.3 + 127.1 pg/ml and in grade 3: 884.3 + 1161.0 pg/ml (statistically non‐significant change). There was a statistically significant Linear Regression between IL‐6 and IL‐8 (P <0.001): IL‐6 = 51.88 pg/ml + (0.092 * IL‐8), r=0.772. Increased activity of the HIF‐1alpha and NF‐κB transcription factors were not observed. Conclusion IL‐6 is a candidate to indicate activity of neovascularization process in PDR. It might be a new molecular therapeutic target to regulate innate immunity response in vitreous.