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Evolution of protein concentration in the rat lens after in vivo exposure to close‐to‐threshold dose ultraviolet radiation
Author(s) -
JING W,
ZHANG J,
LÖFGREN S,
GALICHANIN K,
ZALEBIZADEH N,
YU Z,
SÖDERBERG P
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.245.x
Subject(s) - lens (geology) , in vivo , ultraviolet , ultraviolet radiation , eye lens , irradiation , chemistry , radiation exposure , ophthalmology , biology , medicine , optics , nuclear medicine , radiochemistry , physics , microbiology and biotechnology , nuclear physics
Purpose To investigate the short term cataract development and protein concentration in the rat lens after in vivo close‐to‐threshold dose exposure to ultraviolet radiation (UVR) around 300 nm. Methods Three groups of 10 Sprague Dawley rats were unilaterally exposed to 8 kJ/m2 UVR‐300 nm for 15 minutes, and a forth group of 10 rats was kept without UVR exposure as non‐exposed control animals. The exposed animals were sacrificed at 1, 3 and 7 days after exposure. Both lenses from all animals were extracted and photographed and the intensity of forward light scattering was measured quantitatively. Whole lens was homogenized and protein concentration was determined spectrophotometrically. Results All exposed lenses developed cataract. Lens light scattering increased throughout 7 days after UVR exposure. The difference of protein concentration between exposed lens and contralateral non‐exposed lens was slightly decreased at 1 day after exposure and then gradually normalized back to baseline within 7 day after exposure. Conclusion The in vivo close‐to‐threshold dose UVR induced cataract and protein changes are not paralleled. The altered protein concentration is normalized within 7 days after ultraviolet irradiation.