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Caspase‐3 expression in rat lens after in vivo exposure to UVR‐300 nm
Author(s) -
TALEBI ZADEH N,
GALICHANIN K,
HALLBÖÖK F,
SÖDERBERG P
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.244.x
Subject(s) - significant difference , in vivo , immunohistochemistry , caspase 3 , confidence interval , mean difference , andrology , medicine , apoptosis , ophthalmology , zoology , biology , pathology , programmed cell death , biochemistry , genetics
Purpose To determine the evolution of active caspase‐3 protein expression in albino rat lens after in vivo exposure to low dose UVR‐300 nm by immunohistochemistry. Methods Altogether, 10 Sprague Dawley rats were unilaterally exposed in vivo to 1 kJ/m2 UVR‐300 nm for 15 minutes. At 0.5, 3, 7 and 24 hours after UVR exposure, exposed and contralateral non‐exposed lenses were removed and processed for immunohistochemistry. Three midsagittal sections per lens were stained. Active caspase‐3 labeling was counted and recorded three times in each section. Results Caspase‐3 expression was higher in exposed than in contralateral non‐exposed eyes (expressed as a 95% confidence interval for the mean CI[Mean difference] 0.95 = 13.6 ±0.5). The mean difference between exposed and contralateral non‐exposed lenses was (CI[Mean difference] 0.95) 13.1 ±9.0, 14.3 ±5.6, 18.4 ±1.9 and 8.8 ±7.2 for the 0.5, 3, 7 and 24 hours groups, respectively. There was no difference in expression of caspase‐3 between the 0.5 and 24 hours groups (CI[Mean difference] 0.95 = 4.3 ±7.0). There was a difference between the 3 hours and 7 hours groups (CI[Mean difference] 0.95 = 4.14±2.03). There was a difference when comparing the 0.5 and 24 hours groups versus the 3 and 7 hours groups (CI[Mean difference] 0.95 = 6.21±4.05). Conclusion Expression of caspase‐3 in eye lens increases after UVR exposure. There is a peak of expression between 3 and 7 hours after exposure.