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Kinetics of TP53, CASP3 and GADD45 genes expression in the rat lens after in vivo exposure to subthreshold dose of UV‐B radiation
Author(s) -
GALICHANIN K,
TALEBIZADEH N,
YU Z,
SÖDERBERG P
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.2361.x
Subject(s) - gadd45 , in vivo , downregulation and upregulation , microbiology and biotechnology , messenger rna , lens (geology) , dna damage , gene expression , biology , andrology , chemistry , gene , medicine , dna , biochemistry , genetics , cell cycle , paleontology , cell cycle checkpoint
Purpose Subthreshold dose exposures of UVR‐B over long time accumulate in the lens to cause cataract. The purpose of this study was to investigate kinetics of p53, caspase 3 and gadd45 genes in the rat lens after in vivo exposure to subthreshold UVR‐B around 300 nm. Methods Forty 6‐week‐old female albino Sprague‐Dawley rats were exposed to subthreshold dose (1 kJ/m2) UVR at 300 nm unilaterally for 15 minutes. The animals were sacrificed at 1, 5, 24 and 120 h following the exposure to UVR‐B. p53, caspase 3 and gadd45 mRNA expression of the lenses was analysed by quantitative RT‐PCR. Results p53 is upregulated in 24 h and 120 h groups. Caspase 3 is upregulated in 120 h group. Gadd45 is upregulated in 1 h group. Conclusion p53 mRNA increased expression precedes caspase 3 mRNA increased expression in the rat lens after in vivo UVR‐B exposure. There was initial repair at 1 h showed by increased mRNA expression of DNA repair gene GADD45.