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New cyanine dye for ILM staining
Author(s) -
LAUBICHLER P,
VARJA A,
LANGHALS H,
EIBL K,
KERNT M,
HARITOGLOU C
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.2317.x
Subject(s) - cyanine , staining , fluorescence , biocompatibility , indocyanine green , chemistry , biophysics , pathology , medicine , optics , biology , organic chemistry , physics
Purpose To investigate the biocompatibility and staining properties of a new cyanine dye (DSS: 3,3´‐Di‐(4‐sulfobutyl)‐1,1,1´,1´‐tetramethyl‐di‐1H‐benz[e]indocarbocyanine). Methods Dye concentrations of 0.5%, 0.25% and 0.1% were evaluated (osmolarity between 290 and 295 mOsm). Toxicity was assessed using a colorimetric test measuring the inhibition of ARPE 19 cell, human primary RPE cell and human Müller cell proliferation. Exposure time was 30, 60, 120 and 300 seconds. Indocyanine green (ICG) (0.5%, 0.25% and 0.1%) served as a control. Besides staining of porcine and human lens capsule, internal limiting membrane (ILM)‐staining was assessed by applying 0.25% and 0.5% DSS over the macula in two human post‐mortem eyes. Results The dye DSS did not reveal any toxicity on ARPE‐19, primary human RPE cell and human Müller cells proliferation in all concentrations and exposure times investigated. The absorption maximum is found at 591 nm, the even more bathochromic fluorescence proceeds with a common Stokes’ shift where maxima at 620 and 660 nm with a quantum yield of 32% were found. The fluorescence is sufficiently hypsochromic and the fluorescence quantum yield high enough for an easy visual detection. The contrast and staining properties at the ILM was excellent because of matched optical properties and allowed for a controlled removal of the ILM during surgery. No penetration into deeper retinal layers was noted. Conclusion Our results indicate that this new cyanine dye DSS may represent an alternative for ILM staining due to its matched absorption concerning visibility and fluorescence qualities as well as its good biocompatibility. The dye is superior compared with ICG where there is no matching of the UV/Vis spectra.