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MHC‐II Glial upregulation in contralateral mice retina to experimental glaucoma
Author(s) -
GALLEGO BI,
DE HOZ R,
RAMIREZ AI,
SALAZAR JJ,
ROJAS B,
TRIVINO A,
VALIENTESORIANO FJ,
SALINASNAVARRO M,
RAMIREZ JM
Publication year - 2011
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.2134.x
Subject(s) - retina , retinal , downregulation and upregulation , neuroglia , major histocompatibility complex , mhc class ii , astrocyte , immune system , pathology , chemistry , ophthalmology , biology , microbiology and biotechnology , medicine , immunology , endocrinology , neuroscience , central nervous system , biochemistry , gene
Purpose To analyze the effects of laser‐induced ocular hypertension (OHT) in MHC‐II expression in the glia of eyes with OHT (OHT‐eyes) and contralateral eyes two weeks after lasering. Methods Adult Swiss mice were divided into two groups: naïve (n=6) and lasered (n=6). Retinal wholemounts were immunostained with antibodies against GFAP, Iba‐1 and MHC‐II. Results In the naïve retinas, a weak constitutive MHC‐II expression was scarcely found in some Iba‐1+ cells and rarely in astrocytes. A small dendritiform subpopulation of Iba‐1+ cells, located in the juxtapapillary area and in the marginal region of the retina, had a strong MHC‐II immunoreaction. In comparison naïve results, in the contralateral eye, MHC‐II was expressed in Müller cells and was upregulated in Iba‐1+ cells as well as in the astrocytes. In contralateral macroglia, MHC‐II was preferentially expressed by astrocytes. In OHT‐eyes, Iba‐1+ cells showed MHC‐II immunoreactivity similar to contralateral and no MHC‐II astrocytes were observed; however, MHC‐II expression was upregulated in several groups of Müller cells thoroughout the retina and was preferentially located in the end‐foot of the cells. Conclusion Two weeks of laser induced‐OHT produced macro and microglial retinal changes in MHC‐II expression in both, contralateral and OHT‐eyes. Our results suggest that the gliotic behavior in contralateral untreated eyes could be related to the immune response. On the basis of the glial changes observed, the use of the contralateral eye as a control in experimental unilateral OHT should be reconsidered.