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Influence of donor age, post‐mortem time and cold storage on metabolic profile of human cornea
Author(s) -
Kryczka Tomasz,
Szaflik Jacek P.,
Szaflik Jerzy,
Midelfart Anna
Publication year - 2013
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2011.02271.x
Subject(s) - cornea , metabolite , cold storage , time of death , chemistry , medicine , andrology , ophthalmology , biology , biochemistry , horticulture , emergency medicine
. Purpose: Limited knowledge exists about the influence of donor age and death‐to‐preservation interval (DPI) on the metabolic properties of the cornea. The aim of this study is to investigate the relationship between both factors and metabolite content of the cornea. Methods: Corneas from 15 human donors (age: 41–78 years) were obtained within 16 hrs post‐mortem and kept in cold storage for 8 days. The metabolic profiles of the samples were investigated using high‐resolution, magic angle spinning 1 H nuclear magnetic resonance spectroscopy before and after 8 days of preservation. Results: Twenty‐two metabolites were detected and assigned in the corneal spectra. The significant metabolic differences before and after hypothermic storage were revealed between younger and older donors. DPI‐related significant differences revealed before preservation of the corneas were not displayed after 8 days of cold storage. Conclusions: Age of donor as well as post‐mortem time influences the biochemical properties of the cornea. Cold storage decreases the metabolite differences between the tissues collected at different post‐mortem time.