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Trop 1 gene is overexpressed in pterygia
Author(s) -
CALIENNO R,
CURCIO C,
LANZINI M,
LA SORDA R,
PIANTELLI M,
COLASANTE M,
NUBILE M
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.4164.x
Subject(s) - survivin , vimentin , pathology , immunohistochemistry , cyclin d1 , corneal epithelium , epithelium , biology , antigen , cancer research , medicine , cell , gene , immunology , cell cycle , biochemistry , genetics
Purpose Understanding molecular and biochemical events of pterygium may allow to use less invasive treatments. Several antigens have been investigated to study the physiopathology of this disease. TROP1 protein, a cell adhesion regulatory molecule, and TROP2, a cell surface calcium signal trasducer, are expressed by a large variety of human cancers. Methods The aim of this work is to evaluate TROP1 and 2 protein expression in primitive and recurrent of pterygia. The expression of these antigens was evaluated in limbal crypts (4 cases), corneal epithelium (2 cases), conjunctival melanoma (1 case), normal conjuntiva (4 cases) and pterygia(8 primitive and 4 recurrent). All samples were fixed in formalin, embedded in paraffin and stained by immunohistochemistry. The different expression of TROP1 and 2 was evaluated and correlated with markers normally expressed in pterygia,such as vimentin, ki67, survivin, MMP7, p63, cyclin D1 and p53. Results No expression of TROP1 was observed in corneal epithelium and in limbal crypt, few positive cells were observed in healthy conjuntival epithelium. TROP2 was observed in some cells of limbal crypt, but was not expressed in corneal epithelium, while was positive in conjuntival epithelium. A strong TROP1 and 2 expression was detected in primary pterygia. TROP 1 and 2 positivity correlated with expression of vimentin, ki67, survivin, MMP7, p63, cyclin D1 and p53. Interestingly TROP1 positivity decreased in recurrent pterygia. Conclusion TROP1 and TROP2 antigens can be use as marker also for ocular surface.