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Model of uveitis in rabbit
Author(s) -
EPERON S,
BALASKAS K,
VAUDAUX J,
RODRIGUEZALLER M,
GURNY R,
GUEXCROSIER Y
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.403.x
Subject(s) - chemosis , medicine , uveitis , adjuvant , inflammation , edema , aqueous humor , sensitization , anesthesia , ophthalmology , pharmacology , immunology
Purpose To develop a long‐term experimental model of uveitis using a new easier method for animals. Methods A suspension of desiccated Mycobacterium tuberculosis H37RA was mixed with TiterMax® Gold Adjuvant to produce a water‐in‐oil emulsion. Pigmented rabbits were pre‐sensitized with a s.c. multisite injection (400 ul) of emulsion. Two challenges were performed by intravitreal injection of 100 ul of M. tuberculosis H37RA suspension, under systemic and topical anesthesia. Clinical examinations and aqueous humors punctures under systemic anesthesia were regularly performed till day 71. Hyperemia, chemosis, edema and secretion were clinically scored from 0 to 3. Ocular inflammation was assessed by: a) the clinical score mean (sum of individual scores divided by 4), b) the number of inflammatory cells in aqueous humor (AH) and c) the protein concentration in AH. Results Rabbits presented a nodule at the sites of pre‐sensitization injection. Clinical score of inflammation reached a maximum 3 days after the first intravitreal challenge. The AH contained 835 cells/ ul and protein concentration in AH was 30 mg /ml. During the following days, inflammatory cell number and protein concentration in AH significantly decreased, whereas the clinical score returned to zero. The second intravitreal challenge induced an inflammation; six days after this challenge, 3800 cells/ul and 30 mg of proteins /ml were counted in AH. Inflammation remained high to moderate over 21 days at least. Conclusion We developed a new model of experimental uveitis, whose schedule was simple. The use of TiterMax® Gold Adjuvant showed advantages over other adjuvants. And uveitis could be maintained during 21 days at least.