Premium
Ocular surface reactions following exposure to BAK‐free travoprost/timolol fixed combination, and BAK‐preserved travoprost/timolol and latanoprost/timolol fixed combinations in vivo
Author(s) -
LIANG H,
LABBE A,
PAULY A,
RIANCHO L,
BAUDOUIN C,
BRIGNOLEBAUDOUIN F
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.389.x
Subject(s) - travoprost , latanoprost , timolol , in vivo , ophthalmology , medicine , chemistry , intraocular pressure , biology , microbiology and biotechnology
Purpose To test in vivo the ocular surface reactions of a new formulation of the fixed combination travoprost 0.004%/timolol 0.5% ophthalmic solution (trav/tim BAK‐free) containing the antimicrobial preservative polyquaternium‐1 (PQ, Polyquad®), compared with commercial formulations of the fixed combinations travoprost 0.004%/timolol 0.5% ophthalmic solution (trav/tim BAK, Duotrav®) and latanoprost 0.005%/timolol 0.5% fixed ophthalmic solution (lat/tim BAK, Xalacom®). Methods Phosphate‐buffered saline (PBS), trav/tim BAK‐free, trav/tim BAK, or lat/tim BAK were applied to the eyes of adult male New Zealand albino rabbits (N = 24), 1 drop, 15 times, at 5 min intervals. Ocular surface reactions were assessed at hour 4 and day 1 using slit‐lamp examination, in vivo confocal microscopy (IVCM), conjunctival impression cytology, and immunohistology for detecting CD45+ infiltrating cells and MUC‐5AC‐labeled cells. Results Trav/tim BAK‐free was similar to PBS in regards to the clinical findings, the Draize test for ocular irritation, and epithelial and limbal aspects as evaluated with IVCM. Also, trav/tim BAK‐free and PBS produced no obvious inflammatory infiltration inside and outside the CALT follicles, yielded similar IVCM toxicity scores and CD45+ cell counts, and had normal goblet cell presentation. In contrast, trav/tim BAK and lat/tim BAK induced cytotoxicity in all tests, showing alterations of the ocular surface microstructures and inflammatory infiltration. Conclusion Trav/tim BAK‐free exhibited better tolerance in vivo than trav/tim BAK and lat/tim BAK following repeated frequent instillations in rabbits.