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Hydration and rehydration in camel and bovine cornea
Author(s) -
KHAN F,
ALMUBRAD TM,
AKHTAR S
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.386.x
Subject(s) - sodium , chemistry , cornea , sodium bicarbonate , bicarbonate , swelling , andrology , ophthalmology , medicine , pathology , organic chemistry
Purpose T0 investigate the hydration and rehydration of fresh camel and bovine cornea in sodium salt solutions during 20 minutes, 46 hours and 14 hours. Methods During the 20mins study hydration of fresh corneal stroma was investigated in sodium chloride (NaCl), sodium bicarbonate (NaHCO3), sodium acetate (CH3COONa), sodium thio‐cyanate (NaSCN) and sodium fluoride (NaF) with 2 minutes time interval. During a 46hrs time period, the hydration study was carried out in NaCl (150, 300mM), NaF (150mM) at random intervals. The 14 hrs study was carried out to assess the rehydration of corneal stroma in NaCl (150, 300mM), NaF (150mM) after six hours of drying. Results During the 20 minute swelling studies in the first two minutes the rate of hydration in both camel and bovine corneas was high but gradually reduced in the 2 – 20 minute period. The rate of hydration and level of hydration of camel and bovine cornea was not significantly different from each other in all the strengths of solutions. During the 46 hours swelling studies the rate of initial hydration (0 to 2 hours) of camel and bovine stroma, in all solutions was significantly higher compared to hydration during later hours (2‐46hrs). Camel stromal hydration in150mM NaCl was significantly higher compared to bovine stromal hydration in the same solution during 10 – 24 hrs, and 24 to 46 hrs time period. Rehydration in camel stroma was significantly lower than bovine in 150mM NaF. Conclusion Above study showed that there was no selective affinity for particular ions in camel or bovine corneal stroma. The swelling in camel cornea is higher compared to bovine corneal stroma. This could be due to higher negatively charged KS‐PGs in the stroma. Less rehydration in camel cornea suggests the higher leaching of of PGs from stroma in NaF.