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Comparative toxicology of BAK‐free and BAK‐preserved travoprost/timolol fixed combinations and latanoprost/timolol fixed combination in vitro
Author(s) -
LABBE A,
NAKIB Z,
LIANG H,
BAUDOUIN C,
BRIGNOLEBAUDOUIN F
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.385.x
Subject(s) - travoprost , timolol , latanoprost , viability assay , apoptosis , oxidative stress , in vitro , chemistry , microbiology and biotechnology , biology , pharmacology , medicine , biochemistry , ophthalmology , glaucoma
Purpose To compare, in vitro, the cytotoxicity of a new formulation of travoprost 0.004%/timolol 0.5% fixed combination (FC) ophthalmic solution containing polyquaternium‐1 0.001% (trav/tim BAK‐free) with commercial formulations of travoprost 0.004%/timolol 0.5% FC ophthalmic solution (trav/tim BAK) and latanoprost 0.005%/timolol 0.5% FC ophthalmic solution (lat/tim BAK). Methods Human conjunctival epithelial cells were incubated with PBS, trav/tim BAK‐free, polyquaternium 1 0.001% (PQ), trav/tim BAK with 0.015% BAK, BAK 0.015%, lat/tim BAK with 0.020% BAK, and BAK 0.020%. Cell viability (neutral red, Alamar blue), apoptosis (YO‐PRO‐1, Hoechst 33342), and oxidative stress(H2DCF‐DA, hydroethidine) were assessed. Apoptosis and oxidative stress assays were reported according to cell viability. Results Cells incubated with trav/tim BAK‐free had better viability than cells incubated with lat/tim BAK, trav/tim BAK, BAK 0.015%, and BAK 0.020% (P < 0.0001). Trav/tim BAK‐free produced less apoptosis than trav/tim BAK, lat/tim BAK, BAK 0.015%, and BAK 0.02% (P < 0.0001, YO‐PRO‐1 and Hoechst 33342 relative to cell viability). As assessed with H2DCF‐DA relative to cell viability, PBS, PQ 0.001%, trav/tim BAK‐free, and trav/tim BAK produced significantly less oxidative stress than lat/tim BAK (P < 0.0001). Additionally, as assessed with hydroethidine relative to cell viability, trav/tim BAK‐free produced significantly less oxidative stress than lat/tim BAK (P < 0.0001). Conclusion Trav/tim BAK‐free exhibited significantly less cytotoxicity in vitro than lat/tim BAK and trav/tim BAK. Confirmation is needed in patients with open angle glaucoma or ocular hypertension.