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DHA administration protects antioxidant defenses in human retinal pigmented epithelial cells in high concentration glucose
Author(s) -
ARNAL E,
JOHNSENSORIANO S,
ROMERO FJ
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.378.x
Subject(s) - antioxidant , nitrite , docosahexaenoic acid , retinal , nitric oxide , chemistry , biochemistry , medicine , endocrinology , food science , biology , fatty acid , polyunsaturated fatty acid , organic chemistry , nitrate
Purpose Diabetic retinopathy is a leading cause of visual loss and increased nitric oxide (NO) has been correlated with that. The aim of this study was to evaluate protective properties of Docosahexaenoic acid (DHA) at retinal pigment epithelial (RPE) cells exposed to high glucose concentration. Methods ARPE‐19 were cultured 4 days with normal blood glucose concentration (5,5 mM D‐glucose), followed by exposure to either normal (5,5 mM) or high (45 mM) concentration of D‐glucose during the next 5 days and DHA was added to the culture medium. Four different study groups were obtained: control group (5,5mM D‐glucose) (C), control+DHA group(5,5 mM D‐glucose+6,7 uM DHA)(CDHA), glucose group (45 mM D‐glucose)(G) and glucose+DHA group (45 mM D‐glucose+ 6,7 uM DHA)(GDHA). At the end of the experiment cells were washed, sonicated and lysed and the homogenates criopreserved. The total antioxidant capacity was measured with a commercial kit (Antioxidant Assay Kit, Cayman) and to measured the total nitrites a commercial kit was used (Parameter, Total Nitrite, R&D Systems). Results Antioxidant capacity in Ggroup was decreased significantly (0.97±0.10 mM/mg prot) when compared with the Cgroup (1.52±0.09 mM/mg prot). DHA administration reversed this alteration to normal values in the GDHA group (1.41 ± 0.16 mM/mg prot, p< 0.05 vs control). The total nitrites were significantly elevated in Ggroup (1.09±0.35 umol/L/mg prot) when compared to the Cgroup (0.53±0.08 umol/L/mg prot) and DHA administration reversed this alteration to normal values (0.49±0.12 umol/L/mg prot, p< 0.05 vs G). Conclusion DHA might be useful in protecting diabetic retina, though further studies are needed to better understand this mechanisms.