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Lowering IOP effect, expression of MMP2 in aqueous humour and in trabeculum after pneumotrabeculoplasty in rabbits
Author(s) -
QUINTYN JC,
MASSOUDI D,
BUHANNIC L,
MALECAZE F,
GALLIACY S
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.3157.x
Subject(s) - mmp2 , zymography , mmp9 , aqueous humour , ophthalmology , aqueous humor , matrix metalloproteinase , medicine , surgery , chemistry , downregulation and upregulation , biochemistry , cancer , gene , metastasis
Purpose To assess changes in metalloproteinases (MMP‐2) following pneumotrabeculoplasty in rabbits. Methods Ten New‐Zeland Rabbits were used, 6 for the experiment and 4 for the control group. For the experimental group, the rabbits underwent a single PNT treatment on the both eyes on day 0 (baseline) and after 7 days. The combination of a PNT vacuum application of 60 seconds and a 5 minutes rest period followed by another PNT application constituted a single PNT treatment. All rabbits underwent an intraocular pression before treatment and each week. The whole sample was followed for 21 days. The gelatinolytic activity was analysed by gelatine zymography. This protocol performed twice. Results The intraocular presssion was significantly lower in the experimental group (p< 0.01) at 2 and 3 weeks for the two experiments. Results on gelatinolytic activities, corresponding to pro MMP2, the active form of MMP2 and MMP9, where not clear‐cut. While the first treated group (6 eyes) results show an increase of activity, with a top of activity at day 21, the second experimentation (6 eyes) did not show modification of gelatinolytic activities. Additional studies are required to clarify this point. Conclusion This study confirms the potential of PNT to lower IOP. Additional studies are required to clarify if MMP2 and MMP9 play a role in this reduction, or if the process involves different proteins.