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Oxidative stress in corneal limbal rings
Author(s) -
JOHNSENSORIANO S,
ARNAL E,
ROMERO FJ
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.314.x
Subject(s) - oxidative stress , antioxidant , ophthalmology , antioxidant capacity , chemistry , cornea , medicine , biochemistry
Purpose The aim of this work was to study oxidative stress markers in corneal limbal rings stored in Optisol at different preservation times. Methods Total Antioxidant capacity was measured with a commercial kit (Antioxidant Assay Kit, Cayman). Total nitrites were measured with a commercial kit where the amount of total nitrites was monitored spectofotometrically by reading the absorbance at 540 nm. Moreover, HNE (4‐hydroxy‐2‐nonenal) immunohistoquemistry was performed at 3 µm thick paraffin sections. Results The antioxidant capacity in corneal limbal rings preserved for 7 days were decreased significantly (0.5 ± 0.02 mM/mg prot) when compared to a fresh corneal limbal ring (1.1 ± 0.28 mM/mg prot) * p< 0.05. Moreover, the total nitrites were significantly elevated in corneal limbal rings preserved for 7 days (1.47 ± 0.18 umol/L/mg prot) when compared to fresh corneal limbal rings ( 0.89 ± 0.46 umol/L/mg prot) * p< 0.05. Immunohistoquemistry revealed that there exist an increase in HNE positive cells as conservation time increases. Conclusion Oxidative stress factors increases with time in corneal limbal rings preserved in Optisol.