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Expression and role of aquaporins in diabetic retinopathy
Author(s) -
MOTULSKY E
Publication year - 2010
Publication title -
acta ophthalmologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.534
H-Index - 87
eISSN - 1755-3768
pISSN - 1755-375X
DOI - 10.1111/j.1755-3768.2010.2264.x
Subject(s) - aquaporin , retinal , diabetic retinopathy , in vivo , in vitro , retina , biology , microbiology and biotechnology , cell culture , immunofluorescence , aquaporin 1 , immunology , diabetes mellitus , endocrinology , water channel , antibody , neuroscience , biochemistry , genetics , mechanical engineering , engineering , inlet
Purpose Aquaporins (AQP) are involved in water movements but also in cell proliferation. The aim of our study is to investigate their potential role in diabetic retinopathy Methods In vitro, human and mouse RPE cell line will be grown under different conditions. Expression of AQPs will be investigated by RT‐PCR and Western blot. Immunofluorescence will be used to determine AQP expression on retinal section from normal and NOD diabetic mice, and on on vitreoretinal membranes removed from patients with proliferative retinopathy Results Human ARPE‐19 cells expressed AQP4, while mouse B6‐RPE07 cells expressed AQP1. Hypertonic conditions strongly decrease AQP4 expression in ARPE‐19, but not AQP1 expression in B6‐RPE07 cells. In B6‐RPE07 cells, significantly decrease in AQP1 expression was obtained following TNFα treatment. In normal mice, AQP1 and AQP4 expression were restricted to the photoreceptor layer and to the Müller cells, respectively. Retinal endothelial cells did not express AQP1. No AQP4 expression was detected in RPE cells. AQP1 was strongly expressed by choroidal endothelial cells, rendering difficult the evaluation of AQP1 expression by RPE cells in vivo Conclusion Our preliminary data confirms that blood retinal barrier cells express AQPs in vitro and in vivo. More studies are needed to precise the nature and regulation of this expression in normal and diabetic conditions. We hope that our combined in vitro and in vivo approaches might help to better understand this complex aspect of retinal biology